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Dynabeads® Mouse DC (Dendritic Cell) Enrichment
Use this kit to enrich viable mouse dendritic cells (DC's) from spleen/lymph node cells by negative isolation. The kit depletes T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes. Highly enriched mouse DC's are bead and antibody-free, and can be used in any functional assay. The cells can be further sorted to obtain high purity of specific DC sub-populations.
■Obtain high recovery (80-100%) of viable Lin-CD11c+ DC's of all sup-populations, including DC's positive for CD4, CD8α, B220 and CD19.
■Substantial reduction in sorting time and volume compared to sorting only, thus saving money in addition.
■Pool cells/tissue for easy sample scale -up
Methodology:
An antibody mix towards the non-dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched mouse DC's can be directly sorted into the sup-population of choice or used in any other application.
Capacity: Processes ~1x10^9 cells total
Cell Type: Dendritic Cells
Reactivity: Mouse
Product Size: 5 mL
Target Species: Mouse
Isolation Method: Negative Isolation
Output Viability: >95%
Sample Type (Specific): Lymph Node,
Spleen
Starting Material (Cell #): 1x10^7 cells per isolation
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. |