|
Dynabeads® Untouched™ Human CD4 T Cells Kit
Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.
■High purity, recovery and viability of human untouched CD4+ T cells
■Easy to use and to scale up
■No columns required
Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended
Capacity: Processes ~1x10^9 cells total
Cell Type: T Cells (CD4)
Clonality: Monoclonal
Product Size: 10 mL
Final Product: Cells
Target Species: Human
Isolation Method: Negative Isolation
Output Viability: >98%,
>95%
Number of Reactions: 100 Isolations
Sample Type (Specific): PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
High Throughput Compatibility: Manual Protocols
Shipping Condition: Room Temperature
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
|