ElectroMAX™ DH5α-E™ Competent Cells ElectroMAX™ DH5α-E™ 细胞来源于 DH5α™ 菌株，适合用于电穿孔转化。 它们可用于需要高转化效率的过程，例如生成 cDNA 文库或者用起始量有限的 DNA 进行转化 (1)。 ElectroMAX™ DH5α-E™ 细胞提供以下优点： ■>使用电穿孔获得 1 x 1010 转化子/µg 的效率 ■由于引入了末端A1 突变，大大提高了质粒的产量和品质 ■使用 30 kb 质粒进行高效转化 (2) ■利用 lacZΔM15 对重组克隆进行蓝/白筛选 ■利用 recA1 突变确保插入片段的稳定性 Competent Cell Type: Electrocompetent Species: E. coli Format: Tube(s) Transformation Efficiency: > 1x10^10 Bacterial or Yeast Strain: DH5α™ Plasmid Type: May be used for plasmids > 20 kb Product Size: 0.5 mL Blue-White Screening: Yes Cloning Unstable DNA: Not Suitable for cloning unstable DNA Reduces Recombination: Yes Cloning Methylated DNA: Yes Improves Plasmid Quality: Yes Propagating ccdB Vectors: Not for ccdB vector propagation F' Episome (to make ssDNA): Lacks F' Episome Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA T1 Phage - Resistant (tonA): No High Throughput Compatibility: Not High Throughput-Compatible Shipping Condition: Dry Ice Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.