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Description
Platinum Taq DNA Polymerase is ideal for automatic "hot-start" amplification of DNA fragments with improved specificity over Taq DNA polymerase. It is derived from recombinant Taq DNA polymerase by binding of a thermolabile inhibitor containing monoclonal antibodies to Taq DNA polymerase. During the initial denaturation step of PCR, the inhibitor is denatured and active Taq DNA polymerase is released into the reaction. With Platinum® Taq you'll get:
Improved specificity and yields of PCR compared to Taq DNA polymerase without "hot-start"
PCR products up to 5 kb in size
Room temperature reaction assembly (Figure 1)
Platinum® Blue PCR SuperMix is a PCR master mix containing Platinum® Taq DNA Polymerase, dNTPs, premixed loading/tracking dye, buffer, magnesium and salts. The blue tracking buffer will not inhibit your PCR performance. After PCR cycling, simply load your PCR product directly View Moreonto an agarose gel.
Platinum® Taq PCRx DNA Polymerase is Platinum® Taq DNA Polymerase supplied with the PCRx Enhancer System for optimizing PCR of problematic and/or GC-rich templates. 10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Taq DNA polymerase.
Applications: Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR (1).
Unit Definition: One unit of Platinum® Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonu-cleotide into acid-precipitable material in 30 min. at 74°C. |