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MAX Efficiency® DH10Bac™ Competent Cells
MAX Efficiency® DH10Bac™ 感受态细胞 (MAX Efficiency® DH10Bac™ Competent Cell) 用于产生在 Bac-to-Bac® 杆状病毒表达系统 (Bac-to-Bac® Baculovirus Expression System) 中使用的重组杆状病毒质粒。 DH10Bac™ E. coli 菌株含有的亲代杆状病毒质粒可与供体质粒 pFastBac™ 重组，产生含有目的基因的表达杆状病毒质粒。 亲代杆状病毒质粒含有 lacZα 互补因子，用于阳性重组体的高效蓝/白
筛选。 DH10Bac™ 感受态细胞 (DH10Bac™ Competent Cell) 具有 1 x 108 转化子/µg 的转化效率，以通过位点特异性转座产生重组杆状病毒质粒
Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^8
Bacterial or Yeast Strain: DH10Bac™
Product Size: 5 x 100 µL
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: No
Cloning Methylated DNA: No
Improves Plasmid Quality: No
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible
Shipping Condition: Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Contains:
• MAX Efficiency® DH10Bac™ Chemically Competent Cells: 5 vials, 100 µl each (total of 500 µl)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• SOC Medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature. |