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Taq DNA Polymerase, recombinant
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Life Technologies' Taq DNA Polymerase, you get:
■Your choice of recombinant or native enzyme
■Amplification of PCR products up to 5 kb in size
■An enzyme that is licensed and qualified for PCR
Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.
Source
Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Polymerase: Taq DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 100 units
Exonuclease Activity: 5' - 3'
Shipping Condition: Approved for shipment on Wet or Dry Ice
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.
Contains:
• 20 µl Taq DNA Polymerase (5 U/µl)
• 1.25 ml 10X PCR buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)
• 1 ml Magnesium Chloride (50 mM)
Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly. |