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正品现货|m-HPC Agar|M-HPC琼脂
原理：
Peptone provides nitrogen and carbon as well as other nutri-
ents. The original concentration of 5% gelatin was reduced to
2.5% to avoid problems associated with liquefying gelatin and
spreading colonies.
操作过程：
Water samples should be collected and handled as described in
Standard Methods for the Examination of Water and Waste-
water, Section 9060.
1. The volume to be filtered will vary with the sample. Select a maximum sample size to give 20-200 CFU per filter.
2. Filter the appropriate volume through a sterile 47 mm,
0.45 µm, gridded membrane filter, under partial vacuum.
Rinse funnel with three 20-30 mL portions of sterile dilution
water. Place filter on agar in Petri dish.
3. Place dishes in a close-fitting box or plastic bag containing moistened paper towels.
4. Incubate at 35 ± 0.5°C for 48 hours. Duplicate plates may be incubated at other conditions as desired.
脱水样品制备：1. Suspend 6 g of the powder in 100 mL of purified water. Mix
thoroughly.
2. Heat with frequent agitation and boil for 1 minute to
completely dissolve the powder.
3. Add 1 mL of glycerol.
4. Autoclave at 121°C for 5 minutes. Cool to 45-50°C.
5. Dispense 5 mL portions into 50 × 9 mm Petri dishes.
6. Test samples of the finished product for performance using
stable, typical control cultures.
预期结果：
Count all colonies on the membrane when there are 2 or less colonies per square. For 3-10 colonies per square, count
10 squares and obtain average count per square. For 10-20
colonies per square, count 5 squares and obtain average count
per square. Multiply average count per square by 100 and
divide by the sample volume to give colonies per milliliter. If there are more than 20 colonies per square, record count as > 2,000 divided by the sample volume. Report averaged counts
as estimated colony-forming units. Make estimated counts only
when there are discrete, separated colonies.
用途：
m HPC琼脂用于列举异养有机体在治疗饮用水和其他水样本与低计数通过薄膜过滤。 |