|
Leukemia Inhibitory Factor, recombinant human
Description: Leukemia Inhibitory Factor, recombinant human
Trade Name: Chemicon (Millipore)
Qty/Pk: 10 µg
Product Overview: Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human LIF is a 19.7 kDa protein containing 181 amino acid residues.
Key Applications: Cell Culture
Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary: Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
UniProt Summary: FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SIZE: 202 amino acids; 22008 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
Species: Human
Stem Cell Type: Human Embryonic Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Mesenchymal Stem Cells
Induced Pluripotent Stem Cells
Stem Cell Workflow Stage: Maintenance & Expansion
Differentiation
General Cell Culture
Brand Family: Chemicon
Source: mLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.
Presentation: Liquid in PBS, pH 7.4 and 0.02% Tween 20. No preservativies added.
UniProt Number: P15018
Storage Conditions: Maintain at 2-8°C for 6 - 12 months. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added.
Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
Specific Activity: The activity of human LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of human LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(E8) units/mg, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
Cell Culture Reagent Type: Cytokines & Growth Factors
Gene Symbol: |