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Rat/Mouse Ghrelin (total) ELISA
Description:
Rat/Mouse Ghrelin (total) ELISA
Qty/Pk:
96-well strip plate
Background Information:
Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.
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Applications:
This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.
Key Applications:
ELISA
Application Notes:
Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Species Reactivity:
Rat
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Linearity of Dilution:
Contact Technical Service for this information
Product Name:
Rat/Mouse Ghrelin (total) ELISA
Specificity:
Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%
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Analytes Available:
Ghrelin (total)
UniProt Number:
Q9UBU3
Q9EQX0 (mouse)
Q9QYH7 (rat)
Precision, %:
Intra-assay: Serum: 0.7–1.3% Plasma: 1.1–1.7%
Inter-assay: Serum: 1.8-4.5% Plasma: 2.0-3.2%
Detection Methods:
Fluorescent
Colorimetric
Accuracy:
Plasma: (Active Ghrelin) 86-102%
Plasma: (Des-octanoyl Ghrelin) 95-107%
Serum: (Des-octanoyl Ghrelin) 92-103%
Serum: (Active Ghrelin) 80-103% |