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Human Total Proinsulin ELISA
Description:
Human Total Proinsulin ELISA
Qty/Pk:
96-well strip plate
Background Information:
Proinsulin, the precursor of insulin and C-peptide, is made by the beta cells of the pancreas. This kit measures the intact and intermediate metabolic forms of human proinsulin.
Applications:
This Human Total Proinsulin ELISA is used to measure & quantify Proinsulin levels in Metabolism & Endocrine & Cell Signaling research.
Key Applications:
ELISA
Application Notes:
20 μL sample volume 3 hour assay, room temperature
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
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UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
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Linearity of Dilution:
84–120%
Sensitivity:
0.5 pM
Gene Symbol:
INS
proinsulin
insulin
Product Name:
Human Total Proinsulin ELISA
Standard Curve Range:
2–200 pM
Analytes Available:
Proinsulin
UniProt Number:
P01308
Precision, %:
Inter-assay: 2.9–8.3
Intra-assay: 0.8–8.5
Accuracy:
88–103%
Detection Methods:
Fluorescent
Colorimetric
Entrez Gene Number:
NM_000207.2 |