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Human Leptin "Dual Range" ELISA
Description:
Human Leptin "Dual Range" ELISA
Qty/Pk:
96-well strip plate
Background Information:
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Applications:
This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.
Key Applications:
ELISA
Application Notes:
Room temperature, 3.5 hour assay 25 μL sample size (Sensitive Assay: 50μL)
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
UniProt Summary:
FUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
SIZE: 167 amino acids; 18641 Da
SUBUNIT: Interacts with SIGLEC6.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
Linearity of Dilution:
80–105%
Sensitive Assay 96–125%
Sensitivity:
0.5 ng/mL
Sensitive Assay 0.125 ng/mL
Gene Symbol:
LEP
leptin
OB
obesity
OBS
Product Name:
Human Leptin "Dual Range" ELISA
Standard Curve Range:
0.5–100 ng/mL
Sensitive Assay 0.125–20 ng/mL
Analytes Available:
Leptin
UniProt Number:
P41159
Precision, %:
Inter-assay: 2.6–6.2 Sensitive Assay 1.3–8.6
Intra-assay: 2.6–4.6 Sensitive Assay 1.4–4.9
Accuracy:
94–114%
Sensitive Assay 86–102%
Detection Methods:
Fluorescent
Colorimetric
Entrez Gene Number:
NM_000230.1 |