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Human Insulin ELISA
Description:
Human Insulin ELISA
Qty/Pk:
96-well strip plate
Background Information:
Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.
Applications:
This Human Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research.
Key Applications:
ELISA
Application Notes:
2 hour assay, room temperature Sample ≤ 20 μL serum, plasma, or tissue culture medium
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
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UniProt Summary:
FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
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Linearity of Dilution:
96–112%
Gene Symbol:
INS
proinsulin
insulin
Product Name:
Human Insulin ELISA
Standard Curve Range:
2–200 μU/mL
Analytes Available:
Insulin
UniProt Number:
P01308
Precision, %:
Inter-assay: 9.1–11.4
Intra-assay: 4.6–7.0
Accuracy:
76–103%
Detection Methods:
Fluorescent
Colorimetric
Entrez Gene Number:
NM_000207.2 |