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Rat ESGRO®, 1 million units/1 mL
Description:
Rat ESGRO®
Trade Name:
Chemicon (Millipore)
Qty/Pk:
106 units
Product Overview:
Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.
Applications:
Applications for Rat ESGRO include use as a reagent for the in vitro maintenance of rat ES cells. rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.
Key Applications:
Stem Cell Culture
Application Notes:
Applications for Rat ESGRO® include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et.al. 1998).
Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et.al. 1998).
At this recommended concentration 106 units of ESGRO® is sufficient for 1L of tissue culture media.
Visit www.esgro-lif.com for additional information
Rat LIF is not available for resale.
For research use only; not for use as a diagnostic.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
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UniProt Summary:
FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SIZE: 202 amino acids; 22008 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
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Species:
Rat
Brand Family:
Chemicon
Source:
rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
Presentation:
Purified, sterile liquid. Rat ESGRO® is supplied in liquid form as 106 Units ESGRO® in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability.
Rat ESGRO® is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma.
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Storage Conditions:
Rat ESGRO® is shipped on dry ice. This product is stable for at least 12 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media |