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EmbryoMax® Knockout Kit
Description:
EmbryoMax® Knockout Kit
Trade Name:
Embryomax
Specialty Media
Qty/Pk:
1 kit
Product Overview:
The Knockout Kit contains all the media components that are necessary to generate mouse ES cells used in gene targeting. Gene targeting is a technique that uses homologous recombination between an engineered exogenous DNA fragment and the targeted portion of the mouse genome resulting in a loss of function of the targeted gene. These modified ES cells are then injected into blastocysts where they incorporate into the inner cell mass and contribute to fetal development resulting in the production of "knockout" mice.
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Key Applications:
Stem Cell Culture
Application Notes:
For generating mouse ES cells to be used in gene targeting.
Species Reactivity:
Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Components:
Fetal Bovine Serum (ES-009-B); 500mL (X 1)
DMEM Low Bicarb (SLM-220-B); 500mL (X 7)
PBS w/o Calcium & Magnesium (BSS-1006-B); (X1)
H2O w/ 0.1%Gelatin (ES-006-B ); 500mL (X 1)
Trypsin (SM-2003-C); 100mL (X 1)
100X Nucleosides (ES-008-D); 50mL (X 1)
Non-Essential Amino Acids (TMS-001-C); 100mL (X 1)
beta-Mercaptoethanol (ES-007-E); 20mL (X 2)
100X L-Glutamine (TMS-002-C) and 100X Pen./Strep. (TMS-AB2-C ); 100mL each (X 1 each)
Freezing Media: 2X (ES-002-D) and 1X (S-002-D); 50mL each (X 1 each)
Electroporation Media (ES-003-D); (X 1)
Primary Embryo Fibroblast Feeders: choose between: PMEF-N, PMEF-H, PMEF-CF, PMEF-CFL, PMEF-HL, or PMEF-NL (5 vials; 5 X 106 cells/vial) (X 2)
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Storage Conditions:
Several components with different storage conditions.
Indicated on individual labels.
Packaging:
kit
Product Name:
EmbryoMax® Knockout Kit |