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TNFα ELISPOT Antibody Pair, Human
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PRODUCT FAMILY INFORMATION
Elispot MultiScreenHTS Filter Plates and Antibody Pairs
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen.
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen. The sensitivity is as low as 1 out of 1,000,000 immune cells. This technique is gaining acceptance for monitoring the efficacy of vaccines for cancer and infectious diseases.
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Species Reactivity Key Applications Host Format Antibody Type
H ELISA Mouse Purified Monoclonal Antibody
Description:
TNFα ELISPOT Antibody Pair, Human
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Product Overview:
Tumor Necrosis Factor alpha (TNFα) is a multifunctional cytokine that is secreted by activated macrophages, monocytes, neutrophils, T-cells, and NK cells, and is involved in the regulation of several biological processes including inflammatory responses, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. While under normal conditions, human TNFα primarily functions to regulate immune cells, the overproduction of this cytokine has also been implicated in autoimmune diseases and cancer. The Millipore human TNFα ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of TNFα secreting cells at the single cell level.
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Specificity:
Both the capture and the detection antibodies included in this kit react with human tumor necrosis factor (TNFα).
Molecular Weight:
~17 kDa
Species Reactivity:
Human
Presentation:
The 500 µg purified TNFα capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and should be handled under aseptic conditions. The 100 µg purified TNFα biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.
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Storage Conditions:
Maintain refrigerated at 2-8°C for up to six months from the date of receipt.
Components:
• Human TNFα Capture Antibody, clone MAb1. Quantity: 500 µg.
• Human TNFα Detection Antibody, clone MAb11. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Product Name:
TNFα ELISPOT Antibody Pair, Human
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 kit
Format:
Purified
Host:
Mouse
Applications:
TNFα ELISPOT Antibody Pair, Human is a highly specific mouse monoclonal antibody & that targets TNF & TNF Receptor & has been tested in ELISA.
Packaging:
Sufficient reagents for five, 96-well plates. |