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IL-10 ELISPOT Antibody Pair, Human
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PRODUCT FAMILY INFORMATION
Elispot MultiScreenHTS Filter Plates and Antibody Pairs
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen.
The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen. The sensitivity is as low as 1 out of 1,000,000 immune cells. This technique is gaining acceptance for monitoring the efficacy of vaccines for cancer and infectious diseases.
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Species Reactivity Key Applications Host Format Antibody Type
H ELISA Rat Purified Monoclonal Antibody
Description:
IL-10 ELISPOT Antibody Pair, Human
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Product Overview:
Interleukin 10 (IL-10) is a cytokine that is primarily secreted by T cells and monocytes, and plays an important role in inflammatory and immune responses as it inhibits the synthesis of a number of cytokines, including IFNγ, IL-2, TNFα, and GM-CSF. IL-10 has also been shown to be involved in stimulating the growth of stem cells, mast cells, and thymocytes, in addition to enhancing cytotoxic T cell development and costimulating B cell differentiation and immunoglobulin secretion. The Millipore human IL-10 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-10 secreting cells at the single cell level.
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Specificity:
Both the capture and the detection antibodies included in this kit react with human interleukin -10 (IL-10).
Molecular Weight:
~18 kDa
Species Reactivity:
Human
Presentation:
The 500 µg purified IL-10 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and should be handled under aseptic conditions. The 100 µg purified IL-10 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.
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Storage Conditions:
Maintain refrigerated at 2-8°C for up to six months from the date of receipt.
Components:
• Human IL-10 Capture Antibody, clone JES3-19F1. Quantity: 500 µg.
• Human IL-10 Detection Antibody, clone JES3-12G8. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Product Name:
IL-10 ELISPOT Antibody Pair, Human
Concentration:
Please refer to the Certificate of Analysis for the lot-specific concentration.
Antibody Type:
Monoclonal Antibody
Qty/Pk:
1 kit
Format:
Purified
Host:
Rat
Applications:
IL-10 ELISPOT Antibody Pair, Human is a highly specific rat monoclonal antibody & that targets IL-10 & has been tested in ELISA.
Packaging:
Sufficient reagents for five, 96-well plates. |