|
Goat Anti-Human IgA Antibody, IgG, and IgM, HRP conjugate
Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB, IHC Goat HRP Polyclonal Antibody
Description:
Goat Anti-Human IgA Antibody, IgG, and IgM, HRP conjugate
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Trade Name:
Chemicon (Millipore)
Specificity:
The antibody reacts with human IgG, IgM and IgA. May also react with the light chains of other human immunogloibulins.
Immunogen:
Prepared from purified human IgG, IgM and IgA.
Background Information:
IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15% of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
View All »
Species Reactivity:
Human
Application Notes:
EIA: 1:5,000-1:100,000.
Western blots: 1:5,000-1:200,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by the end user.
Purification Method:
Immunoaffinity purified
Presentation:
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.
Storage Conditions:
Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
View All »
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Key Applications:
ELISA
Western Blotting
Immunohistochemistry
Brand Family:
Chemicon
Product Name:
Goat Anti-Human IgA Antibody, IgG, and IgM, HRP conjugate
Antibody Type:
Polyclonal Antibody
Qty/Pk:
2 mL
Format:
HRP
Host:
Goat
Applications:
Detect Human IgA using this Goat anti-Human IgA Antibody, IgG & IgM, HRP conjugate validated for use in ELISA, WB, IH. |