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Goat Anti-Human IgG Antibody, HRP conjugate
Species Reactivity Key Applications Host Format Antibody Type
H ELISA, WB Goat HRP Polyclonal Antibody
Description:
Goat Anti-Human IgG Antibody, HRP conjugate
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Trade Name:
Chemicon (Millipore)
Product Overview:
Goat anti-Human IgG
Specificity:
The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.
Immunogen:
Prepared from purified human IgG.
Background Information:
IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
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Species Reactivity:
Human
Application Notes:
EIA: 1:5,000-1:100,000.
Western blots: 1:5,000-1:200,000.
Immunohistochemistry: 1:500-1:5,000.
Optimal working dilutions must be determined by the end user.
Purification Method:
Immunoaffinity purified
Presentation:
Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA. Contains no preservative.
Storage Conditions:
Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.
WARNING:
Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
ELISA
Western Blotting
Brand Family:
Chemicon
Product Name:
Goat Anti-Human IgG Antibody, HRP conjugate
Antibody Type:
Polyclonal Antibody
Qty/Pk:
2 mL
Format:
HRP
Host:
Goat
Applications:
Detect Human IgG using this Goat anti-Human IgG Antibody, HRP conjugate validated for use in ELISA & WB. |