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ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, DB, ICC, ChIP Mouse Purified Monoclonal Antibody
Description:
ChIPAb+ Sox-2, clone 6F1.2 - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Specificity:
Recognizes Sox-2, MW: ~34 kDa.
Molecular Weight:
~34 kDa
Immunogen:
Recombinant GST Fusion protein.
Clone:
6F1.2
Isotype:
IgG2bκ
Background Information:
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Sox-2, clone 6F1.2 antibody/primer set includes the Sox-2, clone 6F1.2 antibody, a negative control antibody (purified mouse IgG), and qPCR primers which amplify a 95 bp region within the promoter of the human nanog gene. The Sox-2, clone 6F1.2 antibody and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Sox-2-associated chromatin.
Sox-2 plays a key role in embryonic stem cell development, and has been shown to help stem cells maintain pluripotent potential. It has also been shown to be associated with uncommitted dividing stem cell and precursor cells of the developing CNS.
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Species Reactivity:
Human
Mouse
Species Reactivity Note:
Human, mouse.
Not tested in other species.
Application Notes:
Please refer to the EZ-Magna G ChIP™ (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.
Control:
Includes negative control mouse IgG antibody and primers specific for human nanog promoter region.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from Ntera 2 cells (3 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either a normal mouse IgG, or Anti-Sox-2, clone 6F1.2 antibody and the Magna ChIP™ G Kit (Cat. No. 17-611). Successful immunoprecipitation of Sox-2-associated DNA fragments was verified by qPCR using ChIP Primers, nanog promoter (Figure 1).
Please refer to the EZ-Magna ChIP™ G (Cat. No. 17-409) or EZ-ChIP™ (Cat. No. 17-371) protocol for experimental details.
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Storage Conditions:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
P48431
Entrez Gene Number:
NP_003097
Gene Symbol:
SOX2
ANOP3
MGC2413
MCOPS3
Alternate Names:
Transcription factor SOX-2
SOX2
Components:
Anti-Sox-2, clone 6F1.2 (mouse monoclonal IgG), Part No. CS204373.
One vial containing 100 μg of protein A purified antibody in 140 μL of 0.02 M phosphate buffer pH 7.6 with 0.25 M NaCl containing 0.1% sodium azide, and 30% glycerol. Store at -20°C.
Normal Mouse IgG, Part No. CS200621.
One vial containing 125 μg |