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ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set
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REFERENCES
Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with class III POU factors.
Fumitaka Inoue,Daisuke Kurokawa,Maiko Takahashi,Shinichi Aizawa (2012) Molecular and cellular biology.32
β-Catenin/LEF1 transactivates the microRNA-371-373 cluster that modulates the Wnt/β-catenin-signaling pathway.
A-D Zhou,L-T Diao,H Xu,Z-D Xiao,J-H Li,H Zhou,L-H Qu (2012) Oncogene.31
Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ChIP Mouse Purified Monoclonal Antibody
Description:
ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set
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Trade Name:
Upstate (Millipore)
Product Overview:
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.
The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.
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Specificity:
Recognizes LEF1 (lymphoid enhancer-binding factor 1).
Molecular Weight:
55-57 kDa
Immunogen:
The antibody is made against amino acid residues 1-85 of human LEF1.
Isotype:
IgG1
Species Reactivity:
Human
Mouse
Application Notes:
Western Blot Analysis:
Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LEF1 (1 mg/mL). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Control:
Includes negative control mouse IgG antibody and control primers specific for human c-myc promoter.
Quality Assurance:
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 3x106 HT29 cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Mouse Normal IgG or mouse Anti-LEF1 and the Magna ChIP™ G kit (Cat.# 17-611).
Successful enrichment of LEF1 associated DNA fragments was verified by qPCR using ChIP Primers c-myc flanking the human c-myc promoter that contains LEF1 binding sites (Please see figures).
Please refer to the EZ-Magna ChIP™ G (Cat.# 17-409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.
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Presentation:
Anti-LEF1 (mouse IgG1). 1 vial containing 100 mg of thiophilic and size exclusion chromatography purified mouse IgG1 in 100 mL of phosphate buffered saline with sodium azide. Store at-20°C. The antibody is made against amino acid residues 1-85 of human LEF1 and can recognize human and mouse LEF1. It does not cross-react with TCF-3 or TCF-4.
Normal Mouse IgG. One vial containing 125 μg of normal mouse IgG in 125 μL volume. Store at -20°C.
ChIP primers c-myc. One vial containing 75 mL of 5 μM of each control primer specific for human c-myc promoter. Store at -20°C.
FOR: CCC AAA AAA AGG CAC GGA A
REV: TAT TGG AAA TGC GGT CAT GC
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Storage Conditions:
1 year at -20°C from date of shipment
UniProt Number:
Q9UJU2
Entrez Gene Number:
NM_016269.2
Gene Symbol:
LEF1
DKFZp586H0919
TCF1ALPHA
LEF-1
TCF1-alpha
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Usage Statement:
Unless otherwise stated in our catalog or other |