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Ras Activation ELISA ASSAY Kit
Recommended Replacement for: 17-424
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PRODUCT FAMILY INFORMATION
RAS Antibodies, Kits, Proteins & Plasmids
Millipore’s RAS antibodies, kits, and plasmids are high quality, validated tools for research. These are based on the expertise of Upstate & Chemicon. See below for Millipore’s Ras G-protein products.
Millipore’s RAS antibodies, kits, plasmids and proteins are high quality, validated tools for RAS research. See below for data and references for Millipore’s RAS products. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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Description:
Ras Activation ELISA ASSAY Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
96 assays
Background Information:
Ras proteins function as GDP/GTP-regulated binary switches in signal transduction cascades that can lead to cell growth, proliferation, differentiation, or survival. This Ras superfamily has at least five major branches that include Ras, Rho, Ran, Arf/Sar, Rab. Ras itself has 3 primary isoforms (H-Ras, N-Ras, and K-Ras) that differ in their approximately 20 C-terminal amino acids. They are collectively referred to as Ras. In its active form, Ras is bound to GTP. This causes a conformational change that allow it to interact and bind to several effector molecules, most notably the members of the Raf family, the RalGDS family, and Phosphoinositide 3-kinases (PI3 Kinase). Ras then cleaves GTP to GDP resulting in its inactivation. In its oncogenic, mutated state, Ras is unable to hydrolyze GTP to GDP, thus staying in an active state and activating numerous pathways.
Due to its role as a key regulator of cellular functions and its implications in various cancers, Ras has been a popular target for cancer research and anti-cancer therapeutics for the past two decades for both academic and pharmaceutical research. It was also the first human oncogene identified. Mutations in cellular Ras have been found to be present in a large percentage of all human cancers. More specifically, K-Ras mutations occur frequently in lung, pancreatic, and colon cancers, where as H-Ras mutations are prevalent in bladder, kidney, and thyroid cancers, and N-Ras mutations are associated with melanoma, hepatocellular carcinoma, and leukemia (3). In recent studies, Ras activity has also been implicated in a number of human development defects such as Costello syndrome and Noonan syndrome (4).
One path that the pharmaceutical industry has taken to control Ras and its activity is by attacking what some consider its Achilles’ heel. For its activation, Ras must localize to the plasma membrane, but interestingly, it lacks a transmembrane domain. To achieve this, Ras must first undergo a post-translational modification known as prenylation or geranylation at its C-terminal CAAX motif. These drugs have yet to pass clinical trials though and there is doubt that they will ever be successful in treating tumors associated with Ras activation.
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Key Applications:
ELISA
Cell Function Assay
Species Reactivity:
Human
Mouse
Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
K-Ras
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The t |