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HDAC Assay Kit, fluorometric detection 384 well format
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Description:
HDAC Assay Kit, fluorometric detection 384 well format
Trade Name:
Upstate (Millipore)
Qty/Pk:
384 assays
Product Overview:
The 384 well format kit is a newer version of the tried and true non-radiographic HDAC assay. As in the previous kit, samples are first incubated with the HDAC Assay substrate, allowing deacetylation of the fluorometric substrate. After incubation, the activator solution is added, and releases a fluorophore from the deacetylated substrate or standard. The sample is then put into a microplate reader and fluorescence (i.e free/cleaved acetyl groups) is read.
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Applications:
The 384 well format kit is a newer version of the tried & true non-radiographic HDAC assay. As in the previous kit, samples are first incubated with the HDAC Assay substrate, allowing deacetylation of the fluorometric substrate.
Key Applications:
Enzyme Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
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UniProt Summary:
FUNCTION: SwissProt: Q9UQL6 # Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
SIZE: 1122 amino acids; 121992 Da
SUBUNIT: Interacts with BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with JARID1B.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.TISSUE SPECIFICITY: Ubiquitous.DOMAIN:SwissProt: Q9UQL6 The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm (By similarity).
PTM: Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. & Ubiquitinated. Polyubiquitination however does not lead to its degradation.
SIMILARITY: Belongs to the histone deacetylase family. Type 2 subfamily.
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