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EZ-ChIP™ - Chromatin Immunoprecipitation Kit
Description:
EZ-ChIP™
Trade Name:
EZ-ChIP
Upstate (Millipore)
Qty/Pk:
22 assays
Product Overview:
Chromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.
Features & Benefits:
Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.
Quicker: All reagents to process your samples are included - you don't have to spend valuable time making them.
Greater Reproducibility: Positive and negative control antibodies and PCR primers are included to help validate your results and to troubleshoot your experiments.
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Applications:
Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Components:
DNA Purification Spin Columns and Collection Tubes
ChIP Blocked Protein G Agarose
Anti-RNA Polymerase II
Control PCR Primers
Normal Mouse IgG
Bind, Wash and Elution Reagents
Protease Inhibitor Cocktail II
RNase A
Proteinase K
All required buffers
ChIP Dilution Buffer
Low Salt Immune Complex Wash Buffer
High Salt Immune Complex Wash Buffer
LiCl Immune Complex Wash Buffer
TE Buffer
0.5M EDTA
5M NaCl
SDS Lysis Buffer
1M Tris-HCl, pH 6.5
10X PBS
10X Glycine
1M NaHCO3
Control Primers
20% SDS
Spin Filters
Collection Tubes
Bind Reagent A
Wash Reagent B
Elution Reagent C
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Brand Family:
Upstate
Presentation:
Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
Storage Conditions:
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Packaging:
Kit capacity: 22 chromatin immunoprecipitation assays
Product Name:
EZ-ChIP™ |