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Raf-1 Kinase Assay Kit, Chemiluminescence Detection
Description:
Raf-1 Kinase Assay Kit, Chemiluminescence Detection
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Intended for the assay of recombinant Raf-1 (supplied) kinase activity, with experimental variables as determined by the user. For assay of Raf-1 activity from a cell lysate, we recommend an immunoprecipitation-kinase assay, using anti-Raf-1 antibody (07-396).
Applications:
Intended for the assay of recombinant Raf-1 (supplied) kinase activity, with experimental variables as determined by the user.
Key Applications:
Kinase Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
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UniProt Summary:
FUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
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Brand Family:
Upstate
Gene Symbol:
RAF1
cRaf
Raf-1
c-Raf
RAF
CRAF
C-RAF
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Product Name:
Raf-1 Kinase Assay Kit, Chemiluminescence Detection
Analytes Available:
Raf-1
Quality Assurance:
phosphorylation of MEK1
Components:
Raf-1 (truncated), active (Cat.# 14-352)
MEK1, unactive (Cat.# 14-420)
Magnesium/ATP Cocktail (Cat.# 20-113)
Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) (Cat.# 07-461)
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UniProt Number:
P04049
Detection Methods:
Chemiluminescence
Chemiluminescence
Packaging:
Kit capacity: 50 kinase assays per kit
Entrez Gene Number:
NM_002880.2 |