|
Anti-Cdc42 Immunoblotting Kit
Description:
Anti-Cdc42 Immunoblotting Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
Anti-cdc42 is a mouse monoclonal IgG1 that recognizes cdc42 from human, mouse, rat and dog
Applications:
This Anti-Cdc42 Immunoblotting Kit detects Cdc42 when performing western blots.
Key Applications:
Western Blotting
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
View All »
Entrez Gene Summary:
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
View All »
UniProt Summary:
FUNCTION: SwissProt: P60953 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
SIZE: 191 amino acids; 21311 Da
SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
SIMILARITY: SwissProt: P60953 ## Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
View All »
Brand Family:
Upstate
Presentation:
See Certificate of Analysis for details
Storage Conditions:
Stable for 1 year at -20°C from date of shipment.
Note: Catalog # 12-349, Goat Anti-Mouse IgG, HRP-conjugate is light sensitive, handle accordingly.
Gene Symbol:
CDC42
G25K
CDC42Hs
Product Name:
Anti-Cdc42 Immunoblotting Kit
Quality Assurance:
Routinely evaluated by immunoblot on RIPA lysates from HL-60 cells.
Components:
Anti-cdc42, (mouse monoclonal IgG1), Catalog # 05-542
Immunoblot Blocking Reagent (Cat.# 20-200)
Goat Anti-Mouse IgG, HRP conjugate (Cat.# 12-349)
UniProt Number:
P60953
Host:
Mouse
Packaging:
Kit capacity: 10 immunoblots per kit
Entrez Gene Number:
NM_001039802.1
NM_001791.3
NM_044472.2 |