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Rho Activation Assay Kit
Description:
Rho Activation Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Product Overview:
A GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.
Applications:
PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.
Key Applications:
Affinity Binding Assay
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
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UniProt Summary:
FUNCTION: SwissProt: P62745 # Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
SIZE: 196 amino acids; 22123 Da
SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13.
SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
PTM: Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
SIMILARITY: SwissProt: P62745 ## Belongs to the small GTPase superfamily. Rho family.
MISCELLANEOUS: RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects."
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Brand Family:
Upstate
Gene Symbol:
RHOA
RHO12
Rho12
RhoA
ARHA
ARH12
RHOH12
H12
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Product Name:
Rho Activation Assay Kit
Quality Assurance:
Routinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).
Components:
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)
Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)
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