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HAT Assay Kit
Description:
HAT Assay Kit
Trade Name:
Upstate (Millipore)
Qty/Pk:
1 kit
Applications:
Non-Radioactive HAT Indirect ELISA Assay for the detection of acetyl residues on an acetylated Histone H3 or Histone H4 substrate peptide using an active histone acetyltransferase enzyme.
Key Applications:
ELISA
Application Notes:
Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). See citations for reference link. Use 1- 20μl per assay point in the HAT Reaction Cocktail. Optimal nuclear extract concentration will need to be determined empirically.
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Species Reactivity:
Mouse
Human
Rat
Pig
Horse
Rabbit
Guinea Pig
Hamster
Non-human Primate
Canine
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Entrez Gene Summary:
Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. This gene encodes a histone acetylase that contains A, B, and D motifs, present in many N-acetyltransferases, including those that acetylate substrates other than histones. Alternatively spliced transcript variants that encode different isoforms have been identified for this gene.
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UniProt Summary:
FUNCTION: SwissProt: O14929 # May play a role in telomeric silencing. Acetylates soluble but not nucleosomal H4 at 'Lys-5' and 'Lys-12' and acetylates histone H2A at 'Lys-5'. HAT1 has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG.
SIZE: 419 amino acids; 49513 Da
SUBUNIT: Heteromer of HAT1 and p46/HAT2 subunits.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear in S-phase cells and cytoplasmic.
SIMILARITY: SwissProt: O14929 ## Belongs to the HAT1 family.
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Brand Family:
Upstate
Gene Symbol:
HAT1
Product Name:
HAT Assay Kit
Quality Assurance:
Routinely evaluated in a HAT Assay. Biotinylated Histone H4 or Histone H3 peptides were acetylated with recombinant PCAF (14-309) in the presence of Acetyl-CoA and HAT Assay buffer.
Components:
Streptavidin Coated Microwell Plate (Cat.# 20-183)
Histone H3 Peptide, biotin conjugate (Cat.# 12-403)
Histone H4 Peptide, biotin conjugate (Cat.# 12-405)
HAT Assay Buffer, 5X (Cat.# 20-148)
07-129
TBS, 20X (Cat.# 20-190)
10% BSA in TBS (Cat.# 20-191)
Acetyl-Histone H3 (K9, K14) Peptide biotinylated; Histone H4 (Tetra Ac) Peptide biotinylated; Acetyl-CoA; Goat Anti-Rabbit IgG, HRP-conjugate; TMB (tetramethylbenzidine) Substrate Reagent A and TMB (tetramethylbenzidine) Substrate Reagent B
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UniProt Number:
O14929
Host:
Rabbit
Detection Methods:
Fluorescent
Packaging:
Kit capacity: 96 assays
Entrez Gene Number:
NM_001033085.1
NM_003642.2 |