|
Anti-acetyl-Histone H3
Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, ICC, ChIP-seq, ChIP Rabbit Purified Polyclonal Antibody
Description:
Anti-acetyl-Histone H3 Antibody
Promotional Text:
Special Shipping Offer on Antibodies
100% Performance Guaranteed
Specificity:
Recognizes Histone H3 acetylated at the N-terminus.
Molecular Weight:
~ 17 kDa observed
Epitope:
N-terminus.
Immunogen:
Linear peptide corresponding to human Histone H3 acetylated at the N-terminus.
Modifications:
Acetylation
Background Information:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
Species Reactivity:
Human
Mouse
Rat
Species Reactivity Note:
Demonstrated to react with human, mouse, and rat. Broad species cross-reactivity expected based on sequence similarity.
Application Notes:
Immunocytochemistry Analysis: 1:500 dilution of a representative lot of this antibody detected acetylated Histone H3 in NIH/3T3 and A431 cells.
Chromatin Immunoprecipitation: This antibody has been shown to work for chIP using HeLa extracted chromatin and 5 mg of this antibody.
ChIP-Seq: This antibody has been shown to work for ChIP-Seq in Kanai, A., et. al. (2011). DNA Research 18:379-392.
Control:
HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3
Quality Assurance:
Evaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.
Western Blot Analysis: 0.05 µg/mL of this antibody detected Histone H3 on 10 µg of HeLa acid extract, sodium butyrate treated HeLa cells, and recombinant Histone H3.
Presentation:
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
UniProt Number:
Q16695
Entrez Gene Number:
NP_003484.1
Gene Symbol:
HIST3H3
H3FT
H3t
H3T
H3/g
H3.4
H3/t
Alternate Names:
H3 histone family, member T
histone 3, H3
histone cluster 3, H3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Immunocytochemistry
ChIP-seq
Chromatin Immunoprecipitation (ChIP)
Entrez Gene Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped a |