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Imject KLH Carrier Protein and EDC Conjugation Kit
Soluble KLH and complete EDC conjugation kits for effective peptide-carrier immunogen prep.
Thermo Scientific Imject Mariculture KLH and the accompanying EDC Conjugation Kit are packages of purified keyhole limpet hemocyanin carrier protein that enable simple preparation of highly effective immunogens with peptide antigens.
KLH is the most popular and immunogenic carrier protein used for preparation of peptide antigens for immunization and antibody production. KLH preparations in phosphate buffer are ready for hapten-carrier conjugation via amine-reactive NHS-ester or glutaraldehyde crosslinking. Preparations in MES buffer are optimized for peptide-carrier conjugation via the carboxyl-reactive carbodiimide crosslinker called EDC (or EDAC). The Imject EDC Spin Kit includes ready-to-use units of KLH carrier protein, optimized reaction buffers and trouble-free spin desalting columns for immunogen preparation using the EDC method.
Highlights:
Convenient kit – contains lyophilized mcKLH (in MES buffer), EDC crosslinker and accessories components to easily prepare ready-to-use immunogens for injection
High-yield conjugation – each molecule of KLH contains hundreds of primary amines available for coupling haptens via EDC or NHS ester crosslinkers
Validated quality – purified and stabilized mariculture KLH maintains solubility in aqueous solutions, unlike traditional sources of the carrier protein
Sustainable source – KLH is harvested from select populations of the mollusk Megathura crenulata (keyhole limpet) that are grown in mariculture, not extracted from wild populations
Highly immunogenic – KLH has a high molecular mass (4.5 x 105 to 1.3 x 107 Daltons; aggregates of 350 and 390 kDa subunits) and elicits a stronger immune response than BSA or ovalbumin
Product Details:
Carrier proteins are large, complex molecules capable of stimulating an immune response upon injection. Successful production of antibodies specific to small antigens (i.e., peptides or drug compounds) requires that these haptens be covalently conjugated to a larger, more complex molecule (usually a protein) to make them immunogenic. Carrier proteins are chosen based on immunogenicity, solubility, and whether adequate conjugation with the carrier can be achieved.
Keyhole limpet hemocyanin (KLH) is widely used as a carrier protein for conjugation to haptens and other antigens to make them more immunogenic for the purpose of antibody production. Because of its large mass and complexity, KLH elicits a stronger immune response than other carrier proteins. Because the protein is derived from a mollusk, it is phylogenetically distant from mammalian species and less likely to produce antibodies that will cross-react with typical target samples in assays.
Imject Mariculture KLH (mcKLH) is KLH that has been harvested from limpets grown in mariculture rather than captured from the wild. Imject mcKLH provides the highest possible immunogenicity; it is purified and lyophilized in buffers that optimize its stability and solubility for hapten conjugation. As a large protein, KLH has hundreds of primary amines and carboxyl groups that can be targeted for conjugation with NHS-ester, EDC, and other crosslinking reagents.
Product Details:
Carrier proteins are large, complex molecules capable of stimulating an immune response upon injection. Successful production of antibodies specific to small antigens (i.e., peptides or drug compounds) requires that these haptens be covalently conjugated to a larger, more complex molecule (usually a protein) to make them immunogenic. Carrier proteins are chosen based on immunogenicity, solubility, and whether adequate conjugation with the carrier can be achieved.
Keyhole limpet hemocyanin (KLH) is widely used as a carrier protein for |