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Proteasome 19S Rpn10/S5a subunit (human), (recombinant) (GST-tag)
A subunit of the 26S proteasome, S5a, recognises and binds multi-ubiquitinylated proteins containing chains of at least four ubiquitin moieties. Purified recombinant S5a retains the ability to bind multi-ubiquitinylated proteins in isolation, when immobilised on glutathione-Sepharose, and after being eluted from glutathione-Sepharose, run on SDS-PAGE, and blotted onto nitrocellulose. Human S5a sequence, with an N-terminal fusion of glutathione S-transferase (Schistosoma japonicum), was expressed in E. coli with GST tag at amino terminus.
Product Specification
MW: ~78kDa (observed)
Source: Produced in E. coli.
Formulation: Liquid. In 20mM TRIS, pH 7.5, containing 500mM sodium chloride.
Purity: ≥95% (SDS-PAGE)
Purity Detail: Purified by multi-step chromatography.
Appearance: Colorless liquid.
Activity: Bound to mutliubiquitin chains of length >4 when conjugated to agarose (Prod. No. BML-UW8635).
Application: Useful for the isolation of multi-ubiquitinylated proteins.
Long Term Storage: -80°C |