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Di-ubiquitin (linear)
Linear polyubiquitin chains are useful tools for investigating, amongst other things, the specificity and reactivity of deubiquitinylating enzymes (DUBs) and the recognition and interaction of linear polyubiquitin modified proteins with ubiquitin binding domain (UBDs) containing proteins.
Product Specification
Alternative Name: Ub2
MW: ~17.1kDa
Source: Produced in E. coli.
Formulation: Liquid. In 50mM TRIS, pH 8.0, containing 50mM sodium chloride.
Purity: ≥95% (SDS-PAGE)
Purity Detail: Purified by multistep chromatography.
Application: For use in deubiquitinylating enzyme assays and polyubiquitin binding studies.
Long Term Storage: -20°C
Use/Stability: Stable for at least 12 months after receipt when stored at -20°C.
Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Miscellaneous/General: For use in deubiquitinylating enzyme assays and linear polyubiquitin binding studies.
Linear polyubiquitin chains, in which the C-terminal Gly of one ubiquitin is conjugated to the N-terminal Met of an adjacent ubiquitin, have been shown to be assembled in vitro by the E3 ligase complex LUBAC and ubiquitin binding domains (UBDs) with a preference for linear polyubiquitin have been identified.
A possible role for linear polyubiquitin modification in NF-κB pathway activation has also been reported; suggesting an important role for linear polyubiquitinylation in cellular processes in addition to that of lysine-linked polyubiquitin protein conjugation. |