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Poly-ubiquitin chains (Ub2-16) (K48-linked)
Product Specification
MW: Range
Source: Synthetic. Ubiquitin was polymerized through Lys48 using E1, UbcH5A, and the C-terminal domain of a specific HECT E3.
Formulation: Lyophilized.
Purity Detail: Purified by subtractive anion exchange chromatography.
Application: For use in deubiquitinylating enzyme assays and polyubiquitin binding studies.
Suggested uses:
1. Deubiquitinylating enzyme substrates (general/linkage specific).
2. Investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins
Reconstitution: Note: It is recommended that the product be reconstituted at a moderately high protein concentration (>1mg/mL) and in the presence of buffer. If a more dilute reagent is required, the inclusion of an inert carrier protein (e.g. ovalbumin at 0.5-1.0mg/mL) in the storage buffer is recommended in order to minimise loss of the reagent due to absorption.
Short Term Storage: -20°C
Long Term Storage: -20°C |