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NEDD8 (human), (recombinant) (biotin conjugate)
Product Specification
MW: ~11kDa (based on Bt3-NEDD8 as predominant form present).
Source: Produced in E. coli.
UniProt ID: Q15843
Formulation: Lyophilized solid (from 1:1 acetonitrile:water).
Reconstitution: In order to ensure full solubility it is recommended that the material be dissolved in 5µL dimethyl sulphoxide (DMSO) prior to dilution with the buffer of choice.
Short Term Storage: -20°C
Long Term Storage: -20°C
Use/Stability: Stable for at least 12 months after receipt when stored at -20°C.
Handling: Avoid freeze/thaw cycles.
Miscellaneous/General: Biotinylated NEDD8 is a useful reagent for studying NEDDylation in vitro, where it represents a readily detectable alternative to radio-iodinated NEDD8. Biotinylated NEDD8 is activated by NEDD8 E1, is a competent substrate for the NEDD8 E2 conjugating enzyme UbcH12 and can be conjugated to target proteins in the presence of a suitable NEDD8 E3 ligase. The biotinyl moiety also provides a potential route to affinity purification of conjugated substrates (or thiol ester-linked enzymes) on avidin/streptavidin matrices. NEDD8 contains 9 lysine residues, any/many of which are likely to be biotinylated in this product. These modification sites have not been mapped. Biotinylated NEDD8 is prepared from recombinant human His-tagged NEDD8 (Prod. No. BML-UW9225). |