[D77]Ubiquitin (human), (recombinant) (untagged)
Product Specification
MW: ~8.7 kDa
Source: Produced in E. coli.
UniProt ID: P0CG48
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE)
Purity Detail: Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
Appearance: White solid.
Application: For ubiquitin binding protein interaction and ubiquitin chain assembly studies
Long Term Storage: -20°C
Miscellaneous/General: [D77]Ubiquitin contains an additional aspartic acid residue at its C-terminus. This mutation renders [D77]ubiquitin unable to form protein conjugates via isopeptide bond formation at its C-terminus, but capable of forming poly-ubiquitin chains and interacting with ubiquitin binding proteins. |