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[K63R]Ubiquitin (human), (recombinant) (untagged)
Product Specification
MW: ~8.6 kDa
Source: Produced in E. coli.
UniProt ID: P0CG48
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE) (SDS-PAGE)
Purity Detail: Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
Appearance: White solid.
Application: For production of poly-ubiquitin chains via all lysines except lysine63.
Long Term Storage: -20°C
Miscellaneous/General: [K63R]Ubiquitin, in which Lys63 has been mutated to Arg, is unable to form poly-ubiquitin chains via Lys63 linkages. The ability to form an active thioester at the C-terminus is preserved, allowing [K63R]ubiquitin to be transferred to the lysines of substrate proteins. |