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[K6R]Ubiquitin (human), (recombinant) (untagged)
Product Specification
MW: ~8.6 kDa
Source: Produced in E. coli.
UniProt ID: P0CG48
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE) (SDS-PAGE)
Purity Detail: Purified by perchloric acid treatment of a soluble lysate, followed by gradient elution from a cation exchange column.
Appearance: White solid.
Application: 1. Production of proteins modified by poly-ubiquitin chains, where ubiquitin can be linked via all lysines except Lys6.
2. Investigation of the formation and/or function of specific proteins modified by poly-ubiquitin chains, where ubiquitin can be linked via all lysines except Lys6.
3. Study of the production and/or role of poly-ubiquitin chains linked via all lysines except Lys6.
Long Term Storage: -20°C
Miscellaneous/General: Ubiquitin, in which Lys6has been mutated to Arg, is unable to form poly-ubiquitin chains via Lys6 linkages. The ability to form an active thioester at the C-terminus is preserved, allowing [K6R]ubiquitin to be transferred to the lysines of substrate proteins. |