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Ubiquitin vinyl sulfone, (HA-tag)
For use in the detection and identification of deubiquitinylating enzymes. HA-Ub-VS is a DUB active site directed probe generated by a chemical ligation method, that acts as a potent and irreversible inhibitor of DUBs through covalent modification of the active site and as a specific probe for enzymes with DUB activity. The HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein, facilitates sensitive identification or purification of HA-Ub-VS modified DUBs through recognition by anti-HA antibodies and/or anti-HA-agarose.
Product Specification
Alternative Name: HA-Ub-VS
MW: ~9.8kDa
Source: Synthetic.
UniProt ID: P62988
Formulation: Lyophilized.
Purity: ≥95% (SDS-PAGE)
Application: Suggested uses:
1. Deubiquitinylating enzyme active site-directed probes for detection of novel DUBs.
2. Determination of deubiquitinylating enzyme activity of putative DUBs.
3. Identification of DUBs from biological samples via immunoprecipitation and proteomic methods.
4. Characterisation of deconjugating enzymes for Ub/Ubl specificity.
Reconstitution: Reconstitute with aqueous buffer up to 5mg/ml (~500µM).
Short Term Storage: -80°C
Long Term Storage: -80°C
Use/Stability: Stable for at least 6 months after receipt when stored at -80°C. Once reconstituted store HA-Ub-VS solution at -80ºC, avoid multiple freeze/thaw cycles and use within 1 month.
Handling: Avoid freeze/thaw cycles. |