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Ac-DEVD-AFC
Caspase-3 substrate
Replaces Prod. #: BML-P409
Fluorogenic substrate for caspase-3 (CPP32), with a Km=9.7µM) and related cysteine proteases. Sequence is based on PARP cleavage at Asp216 for caspase-3. Similar to Ac-DEVD-AMC (Prod. No. ALX-260-031) but the AFC fluorophore has a greater Stokes’ shift upon cleavage than AMC. Reaction can be monitored quantitatively or visually using a hand-held long-UV lamp and visualizing a blue to green shift in fluorescence upon cleavage. Ex.: 400nm, Em.: 505nm.
Product Specification
Alternative Name: Caspase-3 substrate (fluorogenic)
Sequence: Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin)
Formula: C30H34F3N5O13
MW: 729.6
CAS: 201608-14-2
Peptide Content: 65-95%
Purity: ≥96% (HPLC)
Appearance: White to off-white powder.
Solubility: Soluble in dimethyl formamide, DMSO or methanol; slightly soluble in water (0.4mg/ml).
Long Term Storage: -20°C
Handling: Protect from light. Keep cool and dry. |