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Goat anti-human IgG (γ-chain specific), (biotin conjugate)
A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality secondary
antibodies are crucial to obtaining good staining of targets that are normally harder to detect, for example due to low
expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection. Secondary antibodies
specifically adsorbed against cross-reacting immunoglobulins will enhance results. ALX-211-502 is recommended for use with
select ALEXIS recombinant fusion proteins containing the Fc portion of human IgG.
Product Specification
Host: Goat
Immunogen: Human IgG.
Application: ELISA
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (frozen sections, paraffin sections)
Western Blot
Note: Use with a streptavidin detection system.
Specificity: Recognizes the heavy chain of human IgG.
Purity Detail: Purified by affinity chromatography on human IgG covalently linked to agarose. Cross-adsorbed with human IgM,
IgD and IgA. Cross-reactivity with IgG from other species has not been evaluated.
Formulation: Liquid. In PBS, pH 7.4, containing 0.1% sodium azide.
Use/Stability: For increased long-term stability, add 40% glycerol to aliquots prior to freezing at -20°C.
Handling: Protect from light. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Short Term Storage: +4°C
Long Term Storage: -20°C |