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Goat anti-mouse IgG (human & mouse Ig adsorbed), (ATTO 647N conjugate)
A frequent cause of high background staining is due to non-specific binding of secondary antibodies. High quality
secondary antibodies are crucial to obtain good staining of targets that are normally harder to detect, for example
due to low expression levels. Interference caused by endogenous immunoglobulins can also result in poor detection.
Secondary antibodies specifically adsorbed against cross-reacting immunoglobulins will enhance results.
Product Specification
Host: Goat
Immunogen: Mouse IgG1.
Application: Immunocytochemistry (1:100)
Optimal conditions must be determined individually for each application.
Specificity: Recognizes the heavy chain of mouse IgG1. Minimally cross-reacts with human immunoglobulins.
Purity Detail: Purified by affinity chromatography on mouse IgG1 covalently limited to agarose. Cross-adsorbed with
mouse IgM, IgG2a, IgG2b, IgG3 and IgA, pooled human sera and purified human paraproteins.
Formulation: Liquid. In PBS, pH 7.4, containing 0.02% sodium azide.
Handling: Protect from light. Do not freeze.
Long Term Storage: +4°C |