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Apg5/Atg5 Antibody
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Product OverviewAntibody Target: Apg5
Alternative Name: Autophagy protein 5, APG5-like , Apoptosis-specific protein, autophagy protein, atg5, atg-5, apg5, apg-5
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 226 of rat Apg5
Accession#: CAJ31281
Gene ID: 365601
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat, pig, bovine
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~37 kDa Apg5/Atg5 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conjugation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conjugation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.
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