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Sphingosine Kinase 1 (SPK1) Antibody
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Product OverviewAntibody Target: Sphingosine Kinase 1 (SK1/SPK1)
Alternative Name: SPK1, SPK-1, SPK 1, sphk, SK1, SK 1
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 180 of mouse Sphingosine Kinase 1
Accession#: CAM17115
Gene ID: 20698
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 42 kDa bands from samples of human, mouse and rat origins. Reactivity to other species has not been tested.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Sphingosine Kinase (SphK) is a conserved lipid kinase that catalyzes the phosphorylation of sphingolipid sphingosine to sphingosine-1-phosphate (SIP). SIP receptors coupled to cell surface G protein to regulate cell growth, survival, motility and inflammatory responses. There are two types of SphK, SphK1 and SphK2. SphK1 is found in the cytosol and migrates to the membrane when activated. SphK1 has been associated with cell growth, prevention of apoptosis and cellular transformation. SphK2 is found mainly in the nucleus and in contrast to SphK1, it enhances the rate of apoptosis.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |