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Insig1 Antibody
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Product OverviewAntibody Target: Insig1
Alternative Name: CL-6 , INSIG1 , MGC1405
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide surrounding amino acid 248 of rat Insig1
Accession#: NP_071787
Gene ID: 64194
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat, bovine, hamster
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~30 kDa InSig-1 and its precursor (~50 kDa) in samples from human, mouse and rat origins. Reactivity to other species has not been tested.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Insulin-induced gene (InSig) localizes in the endoplasmic recticulum(ER) and is highly expressed in the liver and fibroblast cell lines. InSig1 and InSig2 play important roles in the regulation of cholesterol biosynthesis. Sterol induces InSig1 binding to the sterol-sensing domain of SREBP cleavage-activating protein (SCAP). Both InSig1 and Insig2 prevent the export of SCAP from the ER and thus, inhibit cholesterol synthesis by preventing the proteolytic cleavage of SREBPs by the Golgi enzymes.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.
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