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TLR4 Antibody
Product Overview
Antibody Target: TLR4
Alternative Name: TOLL, Toll Like Receptor 4
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residues surrounding amino acids 819 of rat TLR4
Accession#: EDM10505.1
Gene ID: 29260
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-TLR4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: rat
Application: Western blot
Application & Usage: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~92 kDa TLR4 of rat origin. Reactivity to other species has not been tested.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: TLR4 (Toll-like receptor 4) is a mammalain homologue of the Drosophila toll protein. The human TLR4 is a type-1 transmembrane protein containing 799 amino acid residues.
The extracellular domain has leucine-rich repeats and the intracellular domain is similar to that of IL-1 receptor. TLR4 is a receptor for lipopolysaccharide (LPS). In mice that are genetically
tolerant to endotoxin, mutations have been identified in the intracellular domain of TLR4 that abolish LPS responsiveness. TLR4 uses a mechanism similar to that of the IL-1 receptor for signal
transduction that leads to activation of NF-kB.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |