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p53 Antibody
Product Overview
Antibody Target: p53
Alternative Name: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residue surrounding amino acid 375 of human p53
Accession#: EAW90140
Gene ID: 7157
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation:
Purification: Affinity purified
Species Reactivity: human, mouse, rat, dog, rabbit
Application: Western blot
Application & Usage: Western blot analysis (0.5-4 µg/ml). It detects p53 in samples of human, mouse and rat origins. Other applications have not been determined.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans. |