说明：Sequencing primers are single-stranded oligonucleotides with 5'-hydroxyl and 3'-hydroxyl ends. The M13/pUC sequencing primers anneal to the region in the 5'-terminus of the lacZ gene. All primers are supplied as 10 µM aqueous solutions.应用：Sequencing of DNA fragments inserted into the MCS within the lacZ gene of various cloning vectors, such as pUC19, pTZ19R, pTZ57R, M13mp18, pBluescript II.Colony screening by PCR.质量控制：Functionally tested by dideoxy sequencing of pUC19 DNA.