DescriptionRiboRuler Low Range RNA Ladder is a mixture of 7 chromatography purified single-stranded RNA transcripts (100-1000 b long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments. Ladder is available in 1 mM EDTA, pH 6.0.RiboRuler Low Range RNA Ladder is composed of chromatography-purified transcripts what makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes.RiboRuler Low Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase, see protocol, and is ideal for Northern blots.FeaturesSharp bands of uniform intensity.Easy-to-remember band sizes and quantities.Predetermined concentration of ladder bands allows for approximate RNA quantification.Supplied with 2X RNA Loading Dye.Stable for 6 months at -20°C.ApplicationsRNA sizing and quantification on native or denaturing gels.Northern blotting.Quality ControlTested in gel electrophoresis. RNA concentration is determined spectrophotometrically. The absence of ribonucleases confirmed by appropriate tests.StorageStore at -20°C for 6 months or at -70°C for longer periods.注:本产品仅用于生命科学研究实验,不作为任何药物及其他用途。