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Description
O'GeneRuler 1 kb Plus DNA Ladder, ready-to-use, is recommended for sizing and approximate quantification of a wide range of double-stranded DNA onn agarose gels. The ladder is a mixture of chromatography-purified individual DNA fragments.
O'GeneRuler™ 1 kb Plus DNA Ladder, ready-to-use, is premixed with 6X Orange DNA Loading Dye for direct loading on gel. 6X Orange DNA Loading Dye, which contains xylene cyanol FF and orange G. In a 1% agarose gel orange G dye migrates at 50 bp.
The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.
Features
Ideal for both DNA sizing and approximate quantification.
Sharp bands.
Bright reference bands (given in red).
Can be directly loaded and are stable at room temperature for 6 months.
Supplied with loading dye for sample DNA.
O'GeneRuler™ Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF and 10% glycerol.
Storage
Store at room temperature or at 4°C for up to 6 months. For longer periods, store at -20°C.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
6X Orange DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |