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Description
MassRuler Express LR Forward and Reverse DNA ladders are specially designed for fast and reliable DNA quantification in short separation distances under various electrophoresis conditions (see Table 1). In the forward versions, the mass of each DNA fragment is directly proportional to the fragment size, whereas in the reverse versions, the mass is inversely proportional to fragment's size.
The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.
Features
Accurate DNA sizing and quantification – DNA ladder fragments are quantified spectrophotometrically.
Fast and reliable DNA quantification – short separation distances under various electrophoresis conditions (Table 1).
Sharp bands.
Easy-to-remember fragment sizes and quantities.
Ready-to-use – premixed with 6X MassRuler™ DNA Loading Dye for direct loading and room temperature storage.
Supplied with loading dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.
6X MassRuler DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol and 60 mM EDTA.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。 |