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Description
GeneRuler Low Range DNA Ladder is recommended for sizing and approximate quantification of a wide range of double-stranded DNA fragments on agarose or polyacrylamide gels. It is ideal for denaturing polyacrylamide gel electrophoresis. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler Low Range DNA Ladder can be labeled radioactively with T4 Polynucleotide Kinase, see protocol.
The ladder is supplied with 6X DNA Loading Dye.
Features
Ideal for both DNA sizing and approximate quantification.
Ideal for non-denaturing polyacrylamide gel electrophoresis.
Sharp bands.
Bright reference bands (given in red).
Supplied with loading dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |